{"id":9028,"date":"2023-09-20T05:56:15","date_gmt":"2023-09-20T05:56:15","guid":{"rendered":"https:\/\/edukemy.com\/blog\/?p=9028"},"modified":"2023-12-23T09:01:41","modified_gmt":"2023-12-23T09:01:41","slug":"gene-editing-and-gene-therapy-science-and-technology-notes","status":"publish","type":"post","link":"https:\/\/edukemy.com\/blog\/gene-editing-and-gene-therapy-science-and-technology-notes\/","title":{"rendered":"Gene Editing and Gene Therapy-Science and Technology Notes"},"content":{"rendered":"\n
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Gene editing is a powerful and revolutionary biotechnological technique that allows scientists to precisely modify the DNA of living organisms. It enables the addition, deletion, or replacement of specific DNA sequences within the genome, the genetic material that contains an organism’s hereditary information. The most widely used gene editing technology is known as CRISPR-Cas9. <\/p>\n\n\n\n

CRISPR-Cas9, which stands for Clustered Regularly Interspaced Short Palindromic Repeats-CRISPR associated protein 9, is a system borrowed from the bacterial immune system. It has been adapted for use in a wide range of organisms, including plants, animals, and even human cells. The CRISPR-Cas9 gene editing process involves the following steps:<\/p>\n\n\n\n

  1. Guide RNA Design: A small RNA molecule, known as guide RNA, is designed to match the target DNA sequence that needs to be edited.<\/li>
  2. Cas9 Nuclease: The Cas9 protein acts as a pair of “molecular scissors” that can cut the DNA at the specific target site guided by the guide RNA.<\/li>
  3. DNA Modification: Once the Cas9 protein cuts the DNA at the target site, the cell’s natural repair mechanisms come into play. Depending on the desired outcome, the repair process can result in either the deletion, insertion, or replacement of specific DNA segments.<\/li><\/ol>\n\n\n\n